Arturo De LozanneAssociate Professor
Distinguished Teaching Professora.email@example.com
The University of Texas at Austin
Molecular Biosciences, College of Natural Sciences
Austin, TX 78712
Postdoctoral, UT Southwestern Medical Center (1991)
PhD., Stanford University School of Medicine (1988)
B.S., Universidad Autonoma Metropolitana, Mexico (1982)
We are interested in defining the molecular basis of the lysosomal disorder known as the Chediak-Higashi Syndrome. This disease is caused by mutations in a very large gene and result in the loss of a large protein known as Lyst. The size and low abundance of this protein have impaired the detailed analysis of its function and how its absence leads to the dysfunction of lysosomes in CHS patients. We have established a simple model system to study the molecular function of Lyst and closely related proteins in the organism Dictyostelium discoideum. We have shown that the loss of a similar protein in this organism results in the same lysosomal defect. We are currently using the wide array of molecular tools available in this model system to dissect the function of Lyst-related proteins. We hope to use this as a starting point to understand better the human disease and design potential therapies. We are also interested in understanding how eukaryotic cells accomplish the last stage of the cell cycle: cytokinesis. We have identified several proteins important for cytokinesis and have generated knockout mutants lacking these proteins. We then analyze the phenotype of these mutants using a variety of cell biological techniques including imaging of GFP-labeled proteins. One of these proteins called LvsA plays a role in membrane traffic, while another called INCENP binds to chromosomes and microtubules.
Kypri, E., Falkenstein, K., and De Lozanne, A. (2013) Antagonistic control of lysosomal fusion by Rab14 and the Lyst-related protein LvsB. Traffic, 14: 599-609.
Wen, Y., Starvrou I., Bersuker, K., Brady R. J., De Lozanne, A., and O’Halloran, T.J. (2009) AP180- mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles. Mol. Biol. Cell, 20: 4278-4288
Du, F., Edwards, K., Shen, Z., Sun, B., De Lozanne, A., Briggs, S., & Firtel, R. A. (2008) Regulation of Contractile Vacuole Formation and Activity in Dictyostelium. EMBO Journal, 27: 2064-2076.
Li, H., Chen, Q., Kaller, M., Nellen, W., Gräf, R., & De Lozanne, A. (2008) Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinases. Eukaryotic Cell, 7: 894-905.
Kypri, E., Schmauch, C., Maniak, M., and De Lozanne, A. (2007) The BEACH protein LvsB is localized on lysosomes and postlysosomes and limits their fusion with early endosomes. Traffic, 8: 774-783.
Chen, Q., Lakshmikanth, G. S., Spudich, J. A., and De Lozanne, A. (2007) Localization of DdINCENP at the cleavage furrow depends on Kif12, an MKLP1 homologue, and on its interaction with the actin cytoskeleton. Mol. Biol. Cell, 18: 3366-3374.
Chen, Q., Li, H. and De Lozanne, A. (2006) Contractile ring-independent localization of DdINCENP, a protein essential for spindle stability and cytokinesis. Mol. Biol. Cell, 17:779-788.
Wu, W., Yajnik, J., Siano, M., and De Lozanne, A. (2004) Structure-function analysis of the BEACH protein LvsA Traffic, 5:346-355.
De Lozanne, A. (2003) The role of BEACH proteins in Dictyostelium. Traffic, 4: 6-12
2004 President’s Associates Teaching Excellence Award, University of Texas
2005 College of Natural Sciences Teaching Excellence Award, U. of Texas
2006 Jean Holloway Award for Excellence in Teaching, U. of Texas
2006 Faculty Travel Award, U. of Texas
2008 Inducted to the Academy of Distinguished Teachers, U. of Texas
2011 Texas Blazers Faculty Excellence Award
2013 Regents’ Outstanding Teaching Award
BIO 320 - Cell Biology
NSC 110 - Dean's Scholars Seminar
BIO 383K - Current Topics in Cell Biology