Research Summary:

The research in our laboratory focuses on group II intron and related bacterial and organellar reverse transcriptases (RTs), their reverse transcription mechanisms, association with CRISPR-Cas systems, and applications in high-throughput RNA sequencing (RNA-seq), including for the analysis of human cellular, exosomal, and plasma RNAs for RNA diagnostics and liquid biopsy of human diseases. We use a combination of genetic, biochemical, microbiological, and structural approaches. Methods employed include X-ray crystallography and cryo-EM, as well as high-throughput RNA and DNA sequencing and a wide range of bioinformatic and computational methods for analyzing sequence data and biomarker identification in clinical samples, including in currently funded clinical trials.

2020 Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife.

2019 Lentzsch AM, Yao J, Russell R, Lambowitz AM. Template switching mechanism of a group II intron-encoded reverse transcriptase and its implications for biological function and RNA-Seq. Journal of Biological Chemistry.

2019 Temoche-Diaz MM, Shurtleff MJ, Nottingham .MR, Yao J, Fadadu RP, Lambowitz AM, Schekman R. Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. eLife, 8.

2019 Reinsborough CW, Ipas H, Abell NS, Nottingham RM, Yao J, Devanathan SK, Shelton SB, Lambowitz AM, Xhemalce B. BCDIN3D regulates tRNA^His 3’ fragment processing. PLoS Genetics, 15(7).

2019 Belfort M, Lambowitz AM. Group II Intron RNPs and ReverseTranscriptases: From Retroelements to Research Tools. Cold Spring Harbor Perspective in Biology.

2019 Xu H, Yao J, Wu DC, Lambowitz AM. Improved TGIRT-seq methods for comprehensive transcriptome profiling with decreased adapter dimer-formation and bias correction. Scientific Reports, 9(1). 

2018 Mohr G, Silas S, Stamos J, Makarova KS, Markham LM, Yao J, Lucas-Elio P, Sanchez-Amat A, Fire AZ, Koonin EV, et al. A Reverse Transcriptase-Cas1 Fusion Protein Contains a Cas6 Domain Required for Both CRISPR RNA Biogenesis and RNA Spacer Acquisition. Mol. Cell, (72):700-714.

2018 Boivin V, Deschamps-Francoeur G, Couture S, Nottingham RM, Bouchard-Bourelle P, Lambowitz AM. Simultaneous sequencing of coding and noncoding RNA reveals a human transcriptome dominated by a small number of highly expressed noncoding genes. RNA, 24(7):950-965. 

2018 Mohr G, Kang SY, Park SK, Qin Y, Grohman J, Yao J, Stamos JL, Lambowitz AM. A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing. Journal of Molecular Biology, 430(17):2760-2783. 

2018 Wu DC, Yao J, Ho KS, Lambowitz AM, Wilke CO. Limitations of alignment-free tools in total RNA-seq quantification. BMC Genomics, 19(1):510.

2017 Stamos J, Lentzsch AM, Lambowitz AM. Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications. Molecular Cell, 68:926–939. 

2017 Carrell ST, Tang Z, Mohr S, Lambowitz AM, Thorton CA. Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase. Nucleic Acids Research.

2017 Shurtleff MJ, Yao J, Qin Y, Nottingham RM, Temoche-Diaz M, Schekman R, Lambowitz AM. Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. National Academy of Sciences, 114(43). 

2017 Silas S, Makarova KS, Shmakov S, Páez-Espino D, Mohr G, Liu Y, Davidson M, Roux S, Krishnamurthy SR, Fu B, et al. On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires. American Society for Microbiology, 8(4). 

2017 Wu DC, Lambowitz AM. Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching. Scientific Reports, 7(8421). 

2017 Zubradt M, Gupta P, Persad S, Lambowitz AM, Weissman JS, Rouskin S. DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo. Nat Methods, 14(1):75-82. 

2016 Burke JM, Kincaid RP, Nottingham RM, Lambowitz AM, Sullivan CS. DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs. Genes Dev, 30(18):2076-2092. 

2016 Lamech LT, Saoji M, Paukstelis PJ, Lambowitz AM. Structural Divergence of the Group I Intron Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases That Function in RNA Splicing. J Biol Chem, 291(22):11911-27. 

2016 Nottingham RM, Wu DC, Qin Y, Yao J, Hunicke-Smith S, Lambowitz AM. RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase. RNA, 22(4):597-613. 

2016 Qin Y, Yao J, Wu DC, Nottingham RM, Mohr S, Hunicke-Smith S, Lambowitz AM. High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases. RNA, 22(1):111-28. 

2016 Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, Lambowitz AM, Fire AZ. Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein. Science, 351 (6276):aad4234. 

2015 Qin Y, Yao J, Wu DC, Nottingham RM, Mohr S, Hunicke-Smith S, Lambowitz AM. High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases. RNA, Jan;22(1):111-28.

2015 Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, Lambowitz AM, Pan T. Efficient and quantitative high-throughput tRNA sequencing. Nat Methods, Sep;12(9):835-7.

2015 Lambowitz, A.M. and Belfort, M. Mobile bacterial group II introns at the crux of eukaryotic evolution. Microbiol. Spectrum, 3(1) MDNA3-0050-2014. LINK

2015 Truong DM, Hewitt CF, Hanson JH, Cui X, Lambowitz AM. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet, Aug 4;11(8):e1005422.

2015 Shen, P.S., Park, J., Qin, Y., Li, X., Parsawar, K., Larson, M., Cox, J., Cheng, Y., Lambowitz, A.M., Weissman, J.S., Brandman, O., and Frost A. Rqc2p and the 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains, Science, 347, 75-78. LINK

2014 Lamech, L., Mallam, A.L., and Lambowitz, A.M. Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. PLoS Biol., 12, e1002028. LINK

2014 Mallam, A.L., Sidote, D.J., and Lambowitz, A.M. Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase, eLife;3:e04630. LINK

2014 Katibah, G.E., Qin, Y., Sidote, D.J., Yao, J., Lambowitz, A.M.*, and Collins, K*. Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5., Proc. Natl. Acad. Sci. USA 111, 12025-12030, *Co-corresponding authors. LINK

2014 Hong, W., Zhang, J., Feng, Y., Mohr, G., Lambowitz, A.M., Cui, G.-Z., Liu, Y., and Cui, Q. The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons., Biotechnology for Biofuels, 7:80. LINK

2014 Enyeart, P.J., Mohr, G., Ellington, A.D., and Lambowitz A.M., Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis., Mobile DNA 5:2. LINK

2013 Enyeart, P.J., Chirieleison, S.M., Dao, M.N., Perutka, J., Quandt, E.M., Yao, J., Whitt, J.T., Keatinge-Clay, A.T., Lambowitz, A.M., and Ellington, A.D., Generalized bacterial genome editing using mobile group II introns and Cre-lox., Mol. Systems Biol. 9, 685. LINK

2013 Truong, D., Sidote, D., Russell, R., and Lambowitz, A.M., Enhanced group II intron retrohoming in magensium-deficient Escherichia coli via selection of mutations in the ribozyme core., Proc. Natl. Acad. Sci. USA 110, E3800-E3809. LINK

2013 Saldanha, R.J., Pemberton, A., Shiflett, P., Perutka, J., Whitt, J.T., Ellington, A., Lambowitz, A.M., Kramer, R., Taylor, D., and Lamkin, T.J., Rapid targeted gene disruption in Bacillus anthracis., BMC Biotechnology 13, 72. LINK

2013 Mohr, S., Ghanem, E., Smith, W., Sheeter, D., Qin, Y., King, O., Polioudakis, D., Iyer, V.R., Hunicke-Smith, S. Swamy, S., Kuersten, S., and Lambowitz, A.M., Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation sequencing. , RNA 19: 958-970. LINK

2013 Mohr, G., Hong, W., Zhang, J., Cui, G-z., Yang, Y., Qiu, C., Liu, Y-j., Lambowitz, A.M., A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. , PLoS ONE 8(7): e69032. LINK

2013 Russell, R., Jarmoskaite, I., and Lambowitz, A.M., Toward a molecular understanding of RNA remodeling by DEAD-box proteins, RNA Biology 10: 43-54. LINK

2013 Yao, J., Truong, D.M., and Lambowitz, A.M., Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming., PLOS Genetics 9: e1003469. LINK

2012 Mallam A.L., Del Campo M., Gilman B., Sidote D.J., and Lambowitz A.M. , Structural basis for RNA-duplex recognition and unwinding by the DEAD-box helicase Mss116p., Nature 490: 121-125. LINK

2012 White, T.B., and Lambowitz, A.M., The retrohoming of linear group II intron RNAs in Drosophilia melanogaster occurs by both DNA ligase 4-dependent-and-independent mechanisms., PLoS Genetics 8: e1002534. LINK

2011 Lambowitz AM, Zimmerly S., Group II introns: mobile ribozymes that invade DNA., The RNA Worlds, 4th Edition (R.V. Gesteland, T.R. Cech, and J.F. Atkins, Editors), Cold Spring Harbor Press Perspect. Biol. 3(8): a003616. LINK

2011 Mohr, G., Del Campo, M., Turner, K.G., Gilman, B., Wolf, R.Z., and Lambowitz, A.M., High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p., J. Mol. Biol. 413: 952-972. LINK

2011 Mallam, A.L., Jarmoskaite, I., Tijerina, P., Del Campo, M., Seifert, S., Guo, L., Russell, R., and Lambowitz, A.M., Solution structures of DEAD-box RNA chaperones reveal conformational changes and nucleic acid tethering by a basic tail., Proc. Natl. Acad. Sci., USA 108: 12254-12259. LINK

2011 Portatz, J.P., Del Campo, M., Wolf, R., Lambowitz, A.M., and Russell, R., ATP-dependent roles of the DEAD-box protein Mss116p in group II intron splicing in vitro and in vivo., Mol. Biol. 411: 661-679. LINK

2011 Paukstelis, P.J., Chari, N., Lambowitz, A.M., and Hoffman, D., NMR strucure of the C-terminal domain of a tyrosyl-tRNA synthetase that funtions in group I intron splicing., Biochemistry 50: 3816-3826. LINK

2010 Gu, S.-Q., Cui, X., Mou, S., Mohr, S. Yao, J., and Lambowitz A.M., Genetic identification of potential RNA-binding regions in a group II intron-encoded reverse transcriptase., RNA 16: 732-747. LINK

2010 Lambowitz, A.M. and Zimmerly, S., Group II introns: mobile ribozymes that invade DNA. In: The RNA World, 4th Edition (R.F. Gesteland, T.R. Cech, and J.F. Atkins, Editors), Cold Spring Harbor Perspect Biol 1: a003616. LINK

2010 Mohr, G., Ghanem, E., Lambowitz, A.M., Mechanisms used for genomic proliferation by thermophilic group II introns., PLoS Biology 8: e1000391. LINK

2010 Gu, S.-Q., Cui, X., Mou, S., Mohr, S., Yao, J., and Lambowitz, A.M., Genetic identification of potential RNA-binding regions in a group II intron-encoded reverse transcriptase., RNA 16: 732-747

2009 Zhuang, F., Mastroianni, M., White, T.B., and Lambowitz, A.M., Linear group II intron RNAs can retrohome in eukaryotes and may use non-homologus end-joining for cDNA ligation., Proc. Natl. Acad. Sci., USA 106: 18189-18194

2009 Del Campo and Lambowitz, A.M., Structure of the yeast DEAD-box protein Mss116p reveals two wedges that crimp RNA., Mol. Cell 35: 598-609

2009 Del Campo and Lambowitz, A.M., Crystallization and preliminary X-ray diffraction of the DEAD-box protein Mss116p complexed with an RNA oligonucleotide and AMP-PNP., Acta Crystallographica Section F, Structural Biology and Crystallization Communications 65: 832-835

2009 Markov, D.A., Savkina, M., Anikin, M., Del Campo, M. Ecker, K., Lambowitz, A.M., DeGnore, J.P., McAllister, W.T. , Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purfication., Yeast 26: 423-440.

2009 Del Campo, M., Mohr, S., Jiang, Y., Jia, H., Jankowsky, E., and Lambowitz, A.M., Unwinding by local strand separation is critical for the function of DEAD-box proteins as RNA chaperones., J Mol Biol. 389: 674-693

2009 Zhuang, F., Karberg, M., Petrutka, J., and Lambowitz, A.M., EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting., RNA 15: 432-449

2008 Chen, Y., Potratz, J.P., Tijerina, P., Del Campo, M., Lambowitz, A.M., and Russell, R., DEAD-box proteins can completely separate an RNA duplex using a single ATP., Proc Natl Acad Sci U S A. 105: 20203-20208

2008 Vicens Q, Paukstelis PJ, Westhof E, Lambowitz AM, Cech TR, Towards predicting self-splicing and protein-facilitated splicing of group I introns., RNA 14: 2013-2029

2008 Paukstelis, P.J., Lambowitz, A.M., Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity., Proc. Natl. Acad. Sci. USA 105: 6010-6015

2008 Dai, L., Chai, D., Gu, S.Q., Gabel, J., Noskov, S.Y., Blocker, F.J., Lambowitz, A.M., Zimmerly, S., A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase., Mol. Cell 30: 472-485

2008 Zhao, J., Niu, W., Yao, J., Mohr, S., Marcotte, E.M., Lambowitz, A.M., Group II intron protein localiztion and insertion sites are affected by polyphosphate., PLoS Biol. 6: e150

2008 Mastroianni, M., Watanabe, K., White, T.B., Zhuang, F., Vernon, J., Matsuura, M., Wallingford, J., Lambowitz, A.M., Group II intron-based gene targeting reactions in eukaryotes., PLoS ONE 3: e3121

2008 Paukstelis, P.J., Chen, J.H., Chase, E., Lambowitz, A.M., Golden, B.L., Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA., Nature 451: 94-97

2007 Del Campo, M., Tijerina, P., Bhaskaran, H., Mohr, S., Yang, Q., Jankowsk, E., Russell R., and Lambowitz, A.M., Do DEAD-Box proteins promote group II intron splicing without unwinding RNA?, Mol. Cell 28: 159-166

2007 Yang, Q., Del Campo, M., Lambowitz, A.M., and Jankowsky, E., DEAD-box proteins unwind duplexes by local strand separation., Mol. Cell 28: 253-263

2007 Yao, J., and Lambowitz, A.M., Gene targeting in Gram-negative bacteria by use of a mobile group II intron ("targetron") expressed from a broad-host-range vector., Appl. Environ. Microbiol. 73: 2735-2743

2007 Grohman, J.K., Del Campo, M., Tijerina, P., Lambowitz, A.M., and Russell, R., Probing the mechanisms of DEAD-box proteins as general RNA chaperones: the C-terminal domain of CYT-19 mediates general recognition of RNA., RNA 46: 3013-3022

2006 Halls, C., Mohr, S., Del Campo, M., Yang, Q., Jankowsky, E., and Lambowitz, A.M., Involvement of DEAD-box proteins in group I and group II intron splicing. Biochemical characterization of Mss116p, ATP hydrolysis-dependent and -independent mechanisms, and general RNA chaperone activity., J. Mol. Biol. 365: 835-855

2006 Noah, J.W., Park, S., Whitt, J.T., Perutka, J., Frey, W., and Lambowitz, A.M., Atomic force microscopy reveals DNA bending during group II intron ribonucleoprotein particle integration into double-stranded DNA., Biochem. 45: 12424-12435

2006 Yao, J., Zhong, J., Fang, Y., Geisinger, E., Novick, R.P., and Lambowitz, A.M., Use of targetrons to disrupt essential and non-essential genes in Staphylococcus aureus reveals temperature-sensitivity of Ll.LtrB group II intron splicing., RNA 12: 1271-1281

2006 Pyle, A.M., and Lambowitz, A.M., Group II introns: ribozymes that splice RNA and invade DNA. In: The RNA World, 3rd edition., 469-505

2006 Mohr, S., Matsuura, M., Perlman, P.S., and Lambowitz, A.M., A DEAD-box protein by itself promotes group II intron splicing and reverse splicing by acting as an RNA chaperone., Proc. Natl. Acad. Sci. USA 103: 3569-3574

2006 Yao, J., Fang, Y., Geisinger, E., Novick, R.P., and Lambowitz, A.M., Use of targetrons to disrupt essential and non-essential genes in Staphylococcus aureus reveals temperature-sensitivity of Ll.LtrB group II intron splicing., RNA 12: 1271-1281

2005 Zhao, J., and Lambowitz, A.M., A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles., Proc. Natl. Acad. Sci. USA 102: 16233-16140

2005 Smith, D., Zhong, J., Matsuura, M., Lambowitz, A.M., and Belfort, M., Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming., Genes & Devel. 19: 2477-2487

2005 Yao, J., Zhong, J., and Lambowitz, A.M., Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library., Nucl. Acids Res. 33: 3351-3362

2005 Jones, J.P., III, Kierlin, M.N., Coon, R.G., Perutka, J., Lambowitz, A.M., and Sullenger, B.A., Retargeting mobile group II introns to repair mutant genes., Mol. Therapy 11: 687-694

2005 Coros, C.J., Landthaler, M., Piazza, C.L., Beauregard, A., Esposito, D., Perutka, J., Lambowitz, A.M., and Belfort, M., Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment., Mol. Microbio. 56: 509-524

2005 Lambowitz, A.M., Mohr, G., and Zimmerly, S., Group II intron homing endonucleases: ribonucleoprotein complexes with programmable target specificity. In: Nucleic Acids and Molecular Biology., 16: 121-145

2005 Paukstelis, P., Coon, R., Madabusi, L., Nowakowski, J., Monzingo, A., Robertus, J., and Lambowitz, A.M., A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Mol. Cell 17: 417-428

2005 Blocker, F.J.H., Mohr, G., Conlan, L.H., Li, Q., Belfort, M., and Lambowitz, A.M., Domain structure and three-dimensional model of a group II intron-encoded reverse transcriptase., RNA 11: 14-28

2005 Huang, H.-R., Rowe, C.E., Mohr, S., Jiang, Y., Lambowitz, A.M., and Perlman, P.S., The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function., Proc. Natl. Acad. Sci. USA 102: 163-168

2005 Paukstelis, P., Coon, R., Madabusi, L, Nowakowski, J. Monzingo, A., Robertus, J. and Lambowitz, A.M., A tyrosl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Mol. Cell 17: 417-428

2004 Watanabe, K., and Lambowitz, A.M., High-affinity binding site for a group II intron-encoded reverse transcriptase/maturase within a stem-loop structure in the intron RNA., RNA 10: 1433-1444

2004 Lambowitz, A.M., and Zimmerly, S., Mobile group II introns., Annu. Rev. Genet. 38: 1-35

2004 Cui, X., Matsuura, M., Wang, Q., Ma., H., and Lambowitz, A.M., A group II intron-encoded maturase functions preferentially in cis and requires both the reverse transcriptase and X domains to promote RNA splicing., J. Mol. Biol. 340: 211-231

2004 Chen, X., Mohr, G., and Lambowitz, A.M., The Neurospora crassa CYT-18 protein C-terminal RNA-binding domain helps stabilize interdomain tertiary interactions in group I introns., RNA 10: 634-644

2004 Lambowitz, A.M. and Zimmerly, S., Mobile group II introns., Annu. Rev. Genet. 38: 1-35

2004 Perutka, J., Wang, W., Goerlitz, D. and Lambowitz, A.M., Use of computer-designed group II introns to disrupt Escherichia coli DExH/D-box protein and DNA helicase genes., J. Mol. Biol. 336: 421-439

2003 Noah, J.W., and Lambowitz, A.M., Effects of maturase binding and Mg2+ concentration on group II intron RNA folding investigated by UV-cross-linking., Biochemistry 42: 12466-12480

2003 Huang, M.-R., Chao, M.Y., Armstrong, B., Wang, Y., Lambowitz, A.M., and Perlman, P.S., The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing., Mol. Cell. Biol. 23: 8809-8819

2003 Zhong, J., and Lambowitz, A.M., Group II intron mobility using nascent strands at DNA replication forks to prime reverse transcription., EMBO J. 22 : 4555-4565

2003 Munoz-Adelantado, E., San Filippo, J., Mart�nez-Abarca, F., Garc�a-Rodr�guez, F.M., Lambowitz, A.M., and Toro, N., Mobility of the Sinorhizobium meliloti group II intron RmInt1 occurs by reverse splicing into DNA targets, but requires an unknown reverse transcriptase priming mechanism., J. Mol. Biol. 327 : 931-943

2003 Zhong, J., Karberg, M., and Lambowitz, A.M., Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker., Nucl. Acids Res. 31: 1656-1664

2003 Aizawa, Y., Xiang, Q., Lambowitz, A.M., and Pyle, A.M., The pathway for DNA recognition and RNA integration by a group II intron retrotransposon., Mol. Cell 11: 795-805

2003 Frazier, C.L., San Filippo, J., Lambowitz, A.M., and Mills, D.A., Genetic manipulation of Lactococcus lactis using targeted group II introns: generation of stable insertions without selection., Appl. Environ. Microbiol. 69: 1121-1128

2003 Mohr, G., and Lambowitz, A.M., Putative proteins related to group II intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants., Nucl. Acids. Res. 31: 647-652

2002 San Filippo, J., and Lambowitz, A.M., Characterization of the C-terminal DNA-binding/DNA endonuclease region of a group II intron-encoded protein., J. Mol. Biol. 324: 933-951

2002 Mohr, S., Stryker, J., and Lambowitz, A.M., A DEAD-box protein functions as an ATP-dependent RNA chaperone in group I intron splicing., Cell 109: 769-779

2002 Singh, R.N., Saldanha, R.J., D'Souza, L.M., and Lambowitz, A.M., Binding of a group II intron-encoded reverse transcriptase/maturase to its high affinity intron RNA binding site involves sequence-specific recognition and autoregulates translation., J. Mol. Biol. 318: 287-303

2002 Myers, C.A., Kuhla, B., Cusack, S., and Lambowitz, A.M., tRNA-like recognition of group I introns by a tyrosyl-tRNA synthetase., Proc. Natl. Acad. Sci. USA 99 : 2630-2635

2002 Belfort, M., Derbyshire, V., Parker, M.M., Cousineau, B., and Lambowitz, A.M., Mobile introns: pathways and proteins. In: Mobile DNA II (N.L. Craig, R. Craigie, M. Gellert, and A.M. Lambowitz, eds.), 761-783

2001 Matsuura, M., Noah, J.W., and Lambowitz, A.M., Mechanism of maturase-promoted group II intron splicing., EMBO J. 20 : 7259-7270

2001 Karberg, M., Guo, H., Zhong, J., Coon, R., Perutka, J., and Lambowitz, A.M., Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria., Nature 19 : 1162-1167

2001 Dickson, L., Huang, H., Liu, L., Matsuura, M., Lambowitz, A.M. and Perlman, P.S., Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites., Proc. Natl. Acad. Sci. USA 98: 13207-13212

2001 Singh, N.N. and Lambowitz, A.M. , Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference., J. Mol. Biol. 309: 361-386

2001 Caprara, M.G., Myers, C.A., and Lambowitz, A.M., Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) with the group I intron P4-P6 domain. Thermodynamic analysis and the role of metal ions., J. Mol. Biol. 309: 361-386

2001 Mohr, G., Rennard, R., Cherniack, A.D., Stryker, J., and Lambowitz, A.M., Function of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase in RNA splicing. Role of the idiosyncratic N-terminal extension and different modes of interaction with different group I introns., J. Mol. Biol. 307: 75-92

2000 Eskes, R., Liu, L., Ma, H., Chao, M.Y., Dickson, L., Lambowitz, A.M. and Perlman, P.S., Multiple homing pathways used by yeast mitochondrial group II introns., Mol. Cell. Biol. 20: 8432-8446

2000 Chen, X., Gutell, R.R., and Lambowitz, A.M., Function of tyrosyl-tRNA synthetase in splicing group I introns. An induced fit model for binding to the P4-P6 domain based on analysis of mutations at the junction of the P4-P6 stacked helices., J. Mol. Biol. 301: 265-283

2000 Mohr, S., Wanner, L.A., Bertrand, H., and Lambowitz, A.M., Characterization of an unusual tRNA-like sequence found inserted in a Neurospora retroplasmid., Nucl. Acids Res. 28: 1514-1524

2000 Mohr, G., Smith, D., Belfort, M., and Lambowitz, A.M., Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences., Genes & Devel. 14: 559-573

2000 Guo, H., Karberg, M., Long, M., Jones, J.P. III, Sullenger, B., and Lambowitz A.M., Group II introns designed to insert into therapeutically-relevant DNA target sites in human cells., Science 289: 452-457

1999 Wank, H., San Filippo, J., Singh, R.N., Matsuura, M., and Lambowitz, A.M., A reverse transcriptase/maturase promotes splicing by binding at its own coding segment in a group II intron RNA., Mol. Cell 4: 239-250

1999 Saldanha, R., Chen, B., Wank, H., Matsuura, M., Edwards, J., and Lambowitz, A.M., RNA and protein catalysis in group II intron splicing and mobility reactions using purified components., Biochemistry 38: 9069-9083

1999 Zimmerly, S., Moran, J.V., Perlman, P.S., and Lambowitz, A.M. , Group II intron reverse transcriptase in yeast mitochondria. Stabilization and regulation of reverse transcriptase activity by the intron RNA., J. Mol. Biol. 289: 473-490

1999 Lambowitz, A.M., Caprara, M.G., Zimmerly, S., and Perlman, P.S., Group I and group II ribozymes as RNPs: clues to the past and guides to the future. In: The RNA World, Second Edition (R.F. Gesteland, T.R. Cech, and J.F. Atkins, Editors), 451-485

1998 Cousineau, B., Smith, D., Lawrence-Cavanagh, S., Mueller, J.E., Yang, J., Mills, D., Manias, D., Dunny, G., Lambowitz, A.M., and Belfort, M., Retrohoming of a bacterial group II intron: mobility via complete reverse splicing independent of homologous recombination., Cell 54: 451-462

1998 Yang, J., Mohr, G., Perlman, P.S., and Lambowitz, A.M., Group II intron mobility in yeast mitochondria: target DNA-primed reverse transcription activity of aI1 and reverse splicing into DNA transposition sites in vitro., J. Mol. Biol 282: 505-523

1997 Matsuura, M., Saldanha, R., Ma, Hongwen., Wank, H., Yang, J., Mohr, G., Cavanagh, S., Dunny, G.M., Belfort, M., and Lambowitz, A.M., A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron., Genes & Devel. 11: 2910-2924

1997 Guo, H., Zimmerly, S., Perlman, P.S., and Lambowitz, A.M., Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA., EMBO J. 16: 6835-6848

1997 Chen, B., and Lambowitz, A.M., De novo and DNA primer-mediated initiation of cDNA synthesis by the Mauriceville retroplasmid reverse transcriptase involve recognition of a 3' CCA sequence., J. Mol. Biol. 271: 311-332

1997 Chiang, C.-C., and Lambowitz, A.M., The Mauriceville retroplasmid reverse transcriptase initiates cDNA synthesis de novo at the 3' end of tRNAs.The Mauriceville retroplasmid reverse transcriptase initiates cDNA synthesis de novo at the 3' end of tRNAs., Mol. Cell. Biol. 17: 4526-4535

1997 Eskes, R., Yang, J., Lambowitz, A.M., and Perlman, P.S., Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing., Cell 88: 865-874

1997 Wallweber, G.J., Mohr, S., Rennard, R., Caprara, M.G., and Lambowitz, A.M., Characterization of Neurospora mitochondrial group I introns reveals different CYT-18 dependent and independent splicing strategies and an alternative 3' splice site for an intron ORF., RNA 3: 114-131

1996 Caprara, M.G., Lehnert, V., Lambowitz, A.M., and Westhof, E., A tyrosyl-tRNA synthetase recognizes a conserved tRNA-like structural motif in the group I intron catalytic core., Cell 87: 1135-1145

1996 Myers, C.A., Wallweber, G.J., Rennard, R., Kemel, Y., Caprara, M.G., Mohr, G., and Lambowitz, A.M., A tyrosyl-tRNA synthetase suppresses structural defects in the two major helical domains of the group I intron catalytic core., J. Mol. Biol. 262: 87-104

1996 Saldanha, R., Ellington, A., and Lambowitz, A.M., Analysis of the CYT-18 protein binding site at the junction of stacked helices in a group I intron RNA by quantitative binding assays and in vitro selection., J. Mol. Biol. 261: 23-42

1996 Yang, J., Zimmerly, S., Perlman, P.S., and Lambowitz, A.M., Efficient integration of an intron RNA into double-stranded DNA by reverse splicing., Nature 381: 332-335

1996 Caprara, M.G., Mohr, G., and Lambowitz, A.M., A tyrosyl-tRNA synthetase protein induces tertiary folding of the group I intron catalytic core., J. Mol. Biol. 257: 512-531

1996 Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P.S., and Lambowitz, A.M., A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility., Cell 83: 529-538

1995 Zimmerly, S., Guo, H., Perlman, P.S., and Lambowitz, A.M., Group II intron mobility occurs by target DNA-primed reverse transcription., Cell 82: 545-554

1995 Moran, J.V., Zimmerly, S., Eskes, R., Kennell, J.C., Lambowitz, A.M., Butow, R.A., and Perlman, P.S., Mobile group II introns of yeast mitochondrial DNA are novel site-specific retroelements., Mol. Cell. Biol. 15: 2828-2838

1995 Kennell, J.C., Saville, B., Mohr, S., Kuiper, M., Sabourin, J.R., Collins, R.A., and Lambowitz, A.M., The VS catalytic RNA replicates by reverse transcription as a satellite of a retroplasmid., Genes & Devel. 9: 294-303

1995 Lambowitz, A.M., and Chiang, C.-C., The Mauriceville and Varkud plasmids: primitive retroelements found in Neurospora mitochondria. Proceedings Fifth International Congress of Mycology, Vancouver, B.C., Canada, August 1994, Can. J. Botany 73 (Suppl. 1): S173-S179

1995 Chiang, C.-C., Kennell, J.C., Wanner, L.A., and Lambowitz, A.M., A mitochondrial retroplasmid integrates into mtDNA by a novel mechanism involving the synthesis of a hybrid cDNA and homologous recombination., Mol. Cell. Biol. 14: 6419-6432

1995 Saldanha, R.J., Patel, S.S., Surendran, R., Lee, J.C., and Lambowitz, A.M, Involvement of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing. A new method for purifying the protein and characterization of physical and enzymatic properties pertinent to splicing., Biochemistry 34: 1275-1287

1994 Mohr, G., Caprara, M.G, Guo, Q., and Lambowitz, A.M., A tyrosyl-tRNA synthetase can function similarly to an RNA structure in the Tetrahymena ribozyme., Nature 370: 147-150

1994 Kennell, J.C., Wang, H. and Lambowitz, A.M. , The Mauriceville plasmid of Neurospora spp. uses novel mechanisms for initiating reverse transcription in vivo., Mol. Cell. Biol. 14: 3094-3107

1993 Wang, H., and Lambowitz, A.M., The Mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor., Cell 75: 1071-1081

1993 Mohr, G., Perlman, P.S., and Lambowitz, A.M., Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function., Nucl. Acids Res. 21: 4991-4997

1993 Wang, H., and Lambowitz, A.M., Reverse transcription of the Mauriceville plasmid of Neurospora. Lack of RNase H activity associated with the reverse transcriptase and possible use of mitochondrial RNase H., J. Biol. Chem. 268: 18951-18959

1993 Lambowitz, A.M., and Belfort, M., Introns as mobile genetic elements., Annu. Rev. Biochem. 62: 587-622

1993 Kennell, J.C., Moran, J.V., Perlman, P.S., Butow, R.A., and Lambowitz, A.M., Reverse transcriptase activity associated with maturase-encoding group II introns in yeast mitochondria., Cell 73: 133-146

1993 Saldanha, R., Mohr, G., Belfort, M., and Lambowitz, A.M., Group I and group II introns., FASEB J. 7: 15-24

1992 Wang, H., Kennell, J.C., Kuiper, M.T.R., Sabourin, J.R., Saldanha, R., and Lambowitz, A.M., The Mauriceville plasmid of Neurospora crassa. Characterization of a novel reverse transcriptase that begins cDNA synthesis at the 3' end of template RNA., Mol. Cell. Biol. 12: 5131-5144

1992 Guo, Q., and Lambowitz, A.M., A tyrosyl-tRNA synthetase binds specifically to the group I intron catalytic core., Genes & Dev 6: 1357-1372

1992 Mohr, G., Zhang, A., Gianelos, J.A., Belfort, M., and Lambowitz, A.M., The Neurospora CYT-18 protein suppresses defects in the phage T4 td intron by stabilizing the catalytically active structure of the intron core., Cell 69: 483-494

1992 K�mper, U., K�ck, U., Cherniack, A.D., and Lambowitz, A.M., The mitochondrial tyrosyl-tRNA synthetase of Podospora anserina is a bifunctional enzyme active in protein synthesis and RNA splicing., Mol. Cell. Biol. 12: 499-511

1992 Nargang, F.E., Pande, S., Kennell, J.C., Akins, R.A., and Lambowitz, A.M., Evidence that a 1.6 kilobase region of Neurospora spp. mtDNA was derived by insertion of part of the LaBelle mitochondrial plasmid., Nucl. Acids Res. 20: 1101-1108

1992 Turcq, B., Dobinson, K.F., Serizawa, N., and Lambowitz, A.M., A protein required for RNA processing and splicing in Neurospora mitochondria is related to gene products involved in cell cycle protein phosphatase functions., Proc. Natl. Acad. Sci. USA 89: 1676-1680

1991 Mohr, G., and Lambowitz, A.M., Integration of a group I intron into a ribosomal RNA sequence promoted by a tyrosyl-tRNA synthetase., Nature 354: 164-167

1991 Kubelik, A.R., Turcq, B., and Lambowitz, A.M., The Neurospora crassa cyt-20 gene encodes cytosolic and mitochondrial valyl-tRNA synthetases and may have a second function in addition to protein synthesis., Mol. Cell. Biol. 11: 4022-4035

1991 Kittle, J.D., Jr., Mohr, G., Gianelos, J.A., Wang, H., and Lambowitz, A.M., The Neurospora mitochondrial tyrosyl-tRNA synthetase is sufficient for group I intron splicing in vitro and uses the carboxyl-terminal tRNA-binding domain along with other regions., Genes & Devel. 5: 1009-1021

1991 Schulte, U. and Lambowitz, A.M., The LaBelle mitochondrial plasmid of Neurospora intermedia encodes a novel DNA polymerase that may be derived from a reverse transcriptase., Mol. Cell. Biol. 11: 1696-1706

1991 Guo, Q., Akins, R.A., Garriga, G., and Lambowitz, A.M., Structural analysis of the Neurospora mitochondrial large rRNA intron and construction of a mini-intron that shows protein-dependent splicing., J. Biol. Chem. 266: 1809-1819

1990 Lambowitz, A.M., and Perlman, P.S., Involvement of aminoacyl-tRNA synthetases and other proteins in group I and group II intron splicing., TIBS 15: 440-444

1990 Akins, R.A., and Lambowitz, A.M., Analysis of large deletions in the Mauriceville and Varkud mitochondrial plasmids of Neurospora., Current Genetics 18: 365-369

1990 Cherniack, A.D., Garriga, G., Kittle, J.D., Akins, R.A., and Lambowitz, A.M., Function of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing requires an idiosyncratic domain not found in other synthetases., Cell 62: 745-755

1990 Kuiper, M.T.R., Sabourin, J.R., and Lambowitz, A.M., Identification of the reverse transcriptase encoded by the Mauriceville and Varkud mitochondrial plasmids of Neurospora., J. Biol. Chem. 265: 6936-6943

1990 Kubelik, A.R., Kennell, J.C., Akins, R.A., and Lambowitz, A.M., Identification of Neurospora mitochondrial promoters and analysis of synthesis of the mitochondrial small rRNA in wild-type and the promoter mutant [poky]., J. Biol. Chem. 265: 4515-4526

1989 Drygas, M.E., Lambowitz, A.M., and Nargang, F.E., Cloning and analysis of the Neurospora crassa gene for cytochrome c heme lyase., J. Biol. Chem. 264: 17897-17906

1989 Dobinson, K.F., Henderson, M., Kelley, R.L., Collins, R.A., and Lambowitz, A.M., Mutations in nuclear gene cyt-4 of Neurospora crassa result in pleiotropic defects in processing and splicing of mitochondrial RNAs., Genetics 123: 97-108

1989 Kennell, J.C., and Lambowitz, A.M., Development of an in vitro transcription system for Neurospora mtDNA and identification of transcription initiation sites. , Mol. Cell. Biol. 9: 3603-3613

1989 Majumder, A.L., Akins, R.A., Wilkinson, J.G., Kelley, R.L., Snook, A.J., and Lambowitz, A.M., Involvement of tyrosyl-tRNA synthetase in splicing of group I introns in Neurospora crassa mitochondria. Biochemical and immunochemical analysis of splicing activity., Mol. Cell. Biol. 9: 2089-2104

1989 Lambowitz, A.M., Infectious introns (Minireview), Cell 56: 323-326

1989 Lambowitz, A.M., Sabourin, J.R., Bertrand, H., Nickels, R., and McIntosh, L., IImmunological identifcation of the alternative oxidase of Neurospora crassa mitochondria., Mol. Cell. Biol. 9: 1362-1364

1988 Akins, R.A., Kelley, R.L. and, Lambowitz, A.M., Characterization of mutant mitochondrial plasmids of Neurospora spp. that have incorporated tRNAs by reverse transcription., Mol. Cell. Biol. 9: 678-691

1988 Kuiper, M.T.R. and Lambowitz, A.M., A novel reverse transcriptase activity associated with mitochondrial plasmids of Neurospora., Cell 55: 693-704

1988 Akins, R.A., Grant, D.M., Stohl, L.L., Bottorff, D.A., Nargang, F.E. and Lambowitz, A.M., Nucleotide sequence of the Varkud mitochondrial plasmid of Neurospora and synthesis of a hybrid transcript with a 5' leader derived from mitochondrial RNA., J. Mol. Biol. 204: 1-25

1988 Mann, B.J., Akins, R.A., Lambowitz, A.M., and Metzenberg, R.L., The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in the positive regulatory gene nuc-1., Mol. Cell. Biol. 8: 1376-1379

1988 Kuiper, M.T.R., Holtrop, M., Venema, H., Lambowitz, A.M., and de Vries, H., A 3' splice site mutation in a nuclear gene encoding a mitochondrial ribosomal protein in Neurospora crassa., J. Biol. Chem. 263: 2848-2852

1988 Kuiper, M.T.R., Akins, R.A., Holtrop, M., de Vries, H. and Lambowitz, A.M., Isolation and analysis of the Neurospora crassa cyt-21 gene: A nuclear gene encoding a mitochondrial ribosomal protein., J. Biol. Chem. 263: 2840-2847

1987 Akins, R.A. and Lambowitz, A.M., A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereof., Cell 50: 331-345

1987 Paietta, J.V., Akins, R.A., Lambowitz, A.M. and Marzluf, G.A., Molecular cloning of the cys-3+ regulatory gene of Neurospora crassa., Mol. Cell. Biol. 7: 2506-2511

1986 Lambowitz, A.M., Akins, R.A., Kelley, R.L., Pande, S. and, Nargang, F.E. , Mitochondrial plasmids of Neurospora and other filamentous fungi. In: Extrachromosomal Elements in Lower Eukaryotes (R.B. Wickner, A. Hinnebusch, A.M. Lambowitz, I.C. Gunsalus, and A. Hollaender, eds.), 83-92

1986 Medoff, G., Maresca, B., Lambowitz, A.M., Kobayashi, G., Painter, A., Sacco, M. and Carratu, L., A correlation between pathogenicity and temperature-sensitivity of Histoplasma capsulatum., J. Clin. Invest. 78: 1638-1647

1986 Akins, R.A., Kelley, R.L. and Lambowitz, A.M., Mitochondrial plasmids of Neurospora: Integration into mitochondrial DNA and evidence for reverse transcription in mitochondria., Cell 47: 505-516

1986 Garriga, G. and Lambowitz, A.M., Protein-dependent splicing of a group I intron in ribonucleoprotein particles and soluble fractions., Cell 46: 669-680

1986 Garriga, G., Lambowitz, A.M., Inoue, T. and Cech, T.R., Mechanism of recognition of the 5' splice site in self-splicing group I introns., Nature 352: 86-89

1986 Vincent, R.D., Goewert, R., Goldman, W.E., Kobayashi, G.S., Lambowitz, A.M., and Medoff, G., Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms., J. Bacteriol. 165: 813-818

1985 Lambowitz, A.M., Akins, R.A., Garriga, G., Henderson, M., Kubelik, A.R., and Maloney, K.A., Mitochondrial introns and mitochondrial plasmids of Neurospora. In: Achievements and Perspectives in Mitochondrial Research. Volume II: Biogenesis (E. Quagliariello et al., eds.), II: 237-247

1985 Akins, R.A. and Lambowitz, A.M., A general method for cloning Neurospora crassa nuclear genes by complementation of mutants., Mol. Cell. Biol. 5: 2272-2278

1985 Collins, R.A. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Defective splicing of mitochondrial mRNA precursors in nuclear mutant cyt-18-1., J. Mol. Biol. 184: 413-428

1984 Garriga, G. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Self-splicing of a mitochondrial intron in vitro., Cell 39: 631-641

1984 Nargang, F.E., Bell, J.B., Stohl, L.L. and Lambowitz, A.M., The DNA sequence and genetic organization of a Neurospora mitochondrial plasmid suggest a relationship to introns and mobile elements., Cell 38: 441-453

1984 Grant, D.M., Lambowitz, A.M., Rambosek, J.A. and Kinsey, J.A., Transformation of Neurospora with recombinant plasmids containing the cloned glutamate dehydrogenase (am) gene. Evidence for autonomous replication of the transforming plasmid., Mol. Cell. Biol. 4: 2041-2051

1984 Stohl, L.L., Akins, R.A. and Lambowitz, A.M., Characterization of deletion derivative of an autonomously replicating Neurospora plasmid., Nucl. Acids. Res. 12: 6169-6178

1984 Collins, R.A. and Lambowitz, A.M., The physical and genetic map of mitochondrial DNA from Neurospora crassa strain 74-OR23-1A. In: Genetic Maps (S.J. O'Brien, ed.), Vol. III: 274-276

1984 Akins, R.A. and Lambowitz, A.M., The poky mutant of Neurospora contains a four base pair deletion at the 5' end of the mitochondrial small rRNA., Proc. Natl. Acad. Sci. USA 81: 3791-3794

1984 Garriga, G., Bertrand, H., and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Nuclear mutants defective in both splicing and 3' end synthesis of the large rRNA., Cell 36: 623-634

1983 Garriga, G. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria., J. Biol. Chem. 258: 14745-14748

1983 Stohl, L.L. and Lambowitz, A.M., A colony filter hybridization procedure for the filamentous fungus, Neurospora crassa., Anal. Biochem. 134: 82-85

1983 Lambowitz, A.M., Kobayashi, G.S., Painter, A. and Medoff, G., Possible relationship of morphogenesis in pathogenic fungus, Histoplasma capsulatum, to heat shock response., Nature 303: 806-808

1983 Sacco, M., Medoff, G., Lambowitz, A.M., Kumar, B.V., Kobayashi, G.S. and Painter, A., Sulfhydryl-induced respiratory "shunt" pathways and their role in morphogenesis in the fungus, Histoplasma capsulatum., J. Biol. Chem. 258: 8223-8230

1983 Nargang, F.E., Bell, J.B., Stohl, L.L. and Lambowitz, A.M., A family of repetitive GC-rich palindromic sequences found in Neurospora mitochondrial DNA is also found in a mitochondrial plasmid DNA., J. Biol. Chem. 258: 4257-4260

1983 Wollenzien, P.L., Cantor, C.R., Grant, D.M. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Structure of the unspliced 35S precursor ribosomal RNA detected by psoralen cross-linking., Cell 32: 397-407

1983 Stohl, L.L. and Lambowitz, A.M., Construction of a shuttle vector for the filamentous fungus, Neurospora crassa., Proc. Natl. Acad. Sci. USA 80: 1058-1062

1983 Collins, R.A. and Lambowitz, A.M., Structural variations and optional introns in the mitochondrial DNAs of Neurospora strains isolated from nature., Plasmid 9: 53-70

1982 Collins, R.A., Grant, D.M. and Lambowitz, A.M., The physical and genetic map of mtDNA from Neurospora crassa strain 74-OR23-1A. In: Genetic Maps (S.J. O'Brien, ed.), Vol. II: 206-207

1982 Grant, D.M. and Lambowitz, A.M., Mitochondrial rRNA genes. In: The Cell Nucleus (H. Busch and L. Rothblum, eds.), Vol. X Part A: 387-408

1982 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially-assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients., J. Cell Biol. 95: 267-277

1982 Bertrand, H., Bridge, P., Collins, R.A., Garriga, G. and Lambowitz, A.M. , RNA splicing in Neurospora mitochondria. Characterization of new nuclear mutants with defects in splicing the mitochondrial large rRNA., Cell 29: 517-526

1982 Garriga, G., Collins, R.A., Grant, D.M., Lambowitz, A.M., and Bertrand, H., Mitochondrial RNA splicing in Neurospora crassa. In: Mitochondrial Genes (P. Slonimski, P. Borst, and G. Attardi, eds.), 381-390

1982 Stohl, L.L., Collins, R.A., Cole, M.D., and Lambowitz, A.M., Characterization of two new plasmid DNAs found in mitochondria of wild-type Neurospora intermedia strains., Nucl. Acids Res. 10: 1439-1458

1981 Collins, R.A. and Lambowitz, A.M., Characterization of a variant Neurospora crassa mitochondrial DNA which contains tandem reiterations of a 1.9 kb sequence., Curr. Genet. 4: 131-133

1981 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora. Purification of the mitochondrially-synthesized ribosomal protein, S-5., J. Biol. Chem. 256: 7064-7067

1981 Maresca, B., Lambowitz, A.M., Kumar, V.B., Grant, G.A., Kobayashi, G.S. and Medoff, G., Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum., Proc. Natl. Acad. Sci. USA 78: 4596-4600

1981 Collins, R.A., Stohl, L.L., Cole, M.D. and Lambowitz, A.M., Characterization of a novel plasmid DNA found in mitochondria of N. crassa., Cell 24: 443-452

1981 Grimm, M.F., Cole, M.D. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria: Secondary structure of the 35S precursor RNA investigated by digestion with ribonuclease III and by electron microscopy., Biochemistry 20: 2836-2842

1981 Green, M.R., Grimm, M.F., Goewert, R.R., Collins, R.A., Cole, M.D., Lambowitz, A.M., Heckman, J.E., Yin, S. and, RajBhandary, U.L., Transcripts and processing patterns for the ribosomal RNA and transfer RNA region of Neurospora crassa mitochondrial DNA., J. Biol. Chem. 256: 2027-2034

1981 Collins, R.A., Bertrand, H., LaPolla, R.J. and Lambowitz, A.M., A novel extra nuclear mutant of Neurospora with a temperature-sensitive defect in mitochondrial protein synthesis and mitochondrial ATPase., Mol. Gen. Genet. 181: 13-19

1980 Bertrand, H., Collins, R.A., Stohl, L.L., Goewert, R.R. and, Lambowitz, A.M., Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype., Proc. Natl. Acad. Sci. USA 77: 6032-6036

1980 Lambowitz, A.M., Mitochondrial ribosome assembly and RNA splicing in Neurospora crassa., The Organization and Expression of the Mitochondrial Genome (A.M. Kroon and C. Saccone, eds.) 291-300

1980 Boynton, J.E., Gillham, N.W., and Lambowitz, A.M., Biogenesis of chloroplast and mitochondrial ribosomes. In: Ribosomes: Structure, Function and Genetics (G. Chamblis, G.R. Craven, J. Davies, K. Davis, L. Kahan and M. Nomura, ed.) , 903-950

1979 Mannella, C.A., Goewert, R.R. and Lambowitz, A.M., Characterization of variant Neurospora crassa mitochondrial DNAs which contain tandem reiterations., Cell 18: 1197-1207

1979 Mannella, C.A. and Lambowitz, A.M., Unidirectional gene conversion associated with two insertions in Neurospora crassa mitochondrial DNA., Genetics 93: 645-654

1979 LaPolla, R.J. and Lambowitz, A.M., The binding of mitochondrial ribosomal proteins to a mitochondrial ribosomal precursor RNA containing a 2.3 kb intron., J. Biol. Chem. 254: 11746-11750

1979 Grimm, M.F. and Lambowitz, A.M., The 32 S RNA of Neurospora crassa mitochondria is not a precursor of the mitochondrial rRNAs., J. Mol. Biol. 134: 667-672

1979 Collins, R.A., Bertrand, H., LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora crassa: Mutants with defects in mitochondrial ribosome assembly., Mol. Gen. Genet. 177: 73-84

1979 Maresca, B., Lambowitz, A.M., Kobayashi, G.S. and Medoff, G., Respiration in the yeast and mycelial phases of Histoplasma capsulatum., J. Bacteriol. 138: 647-649

1979 Mannella, C.A., Collins, R.A., Green, M.R. and Lambowitz, A.M., Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa., Proc. Natl. Acad. Sci. USA 76: 2635-2639

1979 Lambowitz, A.M., LaPolla, R.J. and Collins, R.A., Mitochondrial ribosome assembly in Neurospora. Two-dimensional gel analysis of mitochondrial ribosomal proteins., J. Cell Biol. 82: 17-31

1979 Mannella, C.A., Pittenger, T.H. and Lambowitz, A.M., Transmission of mitochondrial DNA in Neurospora crassa sexual crosses., J. Bacteriol. 137: 1449-1451

1979 Lambowitz, A.M., Preparation and analysis of mitochondrial ribosomes., Meth. Enzymol. 59: 421-433

1978 Lambowitz, A.M. and Zannoni, D., Cyanide-insensitive respiration in Neurospora; genetic and biophysical approaches. In: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher et al., ed.), 283-291

1978 Mannella, C.A. and Lambowitz, A.M., Interaction of wild-type and poky mitochondrial DNA in heterokaryons of Neurospora., Biochem. Biophys. Res. Commun. 80: 673-679

1977 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora crassa; chloramphenicol inhibits the maturation of small ribosomal subunits., J. Mol. Biol. 116: 189-205

1976 Lambowitz, A.M., Chua, N.H. and Luck, D.J.L., Mitochondrial ribosome assembly in Neurospora crassa; preparation of ribosomal precursor particles, site of synthesis of mitochondrial ribosomal proteins, and studies on the poky mutant., J. Biol. Chem. 107: 223-253

1976 Lambowitz, A.M. and Luck, D.J.L., Studies on the poky mutant of Neurospora crassa; fingerprint analysis of mitochondrial ribosomal RNA., J. Biol. Chem. 251: 3081-3095

1975 Lambowitz, A.M. and Luck, D.J.L., Methylation of mitochondrial RNA species in wild-type and poky strains of Neurospora crassa., J. Mol. Biol. 96: 207-214

1974 Bonner, W.D., Jr., Lambowitz, A.M. and Wikstrom, M.K.F. , On the lack of ATP induced midpoint potential shift in cytochrome b-566 in plant mitochondria. In: Dynamics of Energy-Transducing Membranes (L. Ernster, R.W. Estabrook, and E.C. Slater, eds.), 195-200

1974 Lambowitz, A.M. and Bonner, W.D., Jr., The b cytochromes of plant mitochondria. In: Dynamics of Energy-Transducing Membranes (L. Ernster, R.W. Estabrook, and E.C. Slater, eds.), 77-92

1974 Wikstrom, M.K.F. and Lambowitz, A.M., The interaction of redox mediators with the second phosphorylation site; significance for the cytochrome bT hypothesis., FEBS Lett. 40: 149-153

1974 Lambowitz, A.M. and Bonner, W.D., Jr., The mitochondrial b-cytochromes of the wild-type and poky strains of Neurospora crassa; evidence for a component reduced only by dithionite., J. Biol. Chem. 249: 2886-2890

1974 Lambowitz, A.M., Bonner, W.D., Jr. and Wikstrom, M.K.F., On the lack of ATP induced midpoint potential shift for cytochrome b-566 in plant mitochondria., Proc. Natl. Acad. Sci. USA 71: 1183-1187

1974 Lambowitz, A.M. and Bonner, W.D., Jr., The b-cytochromes of plant mitochondria; a spectrophotometric and potentiometric study., J. Biol. Chem. 249: 2428-2440

1973 Lambowitz, A.M. and Bonner, W.D., Jr., The effect of antimycin on the b-cytochromes of plant mitochondria; oxidation-reduction behavior of cytochrome b-566., Biochem. Biophys. Res. Commun. 52: 703-711

1972 Lambowitz, A.M. and Slayman, C.W., Effect of pyrrolnitrin on electron transport and oxidative phosphorylation in mitochondria isolated from Neurospora crassa., J. Bacteriol. 112: 1020-1022

1972 Lambowitz, A.M., Smith, E.W. and Slayman, C.W., Oxidative phosphorylation in Neurospora mitochondria; studies on wild type, poky, and chloramphenicol-induced wild type., J. Biol. Chem. 247: 4859-4865

1972 Lambowitz, A.M., Smith, E.W. and Slayman, C.W., Electron transport in Neurospora mitochondria; studies on wild type and poky., J. Biol. Chem. 247: 4850-4858

1972 Lambowitz, A.M., Slayman, C.W., Slayman, C.L. and Bonner, W.D., Jr., The electron transport components of the wild-type and poky strains of Neurospora crassa., J. Biol. Chem. 247: 1536-1545

1971 Lambowitz, A.M. and Slayman, C.W., Cyanide-resistant respiration in Neurospora crassa., J. Bacteriol. 108: 1087-1096

Basil O'Connor Starter Research Grant from National Foundation-March of Dimes, 1977-1980; NIH Merit Award, 1993; American Academy of Arts and Sciences, 1995; UT Cooperative Society Award for Best Research Paper, 2000; Fellow, American Association for the Advancement of Science, 2001; American Academy of Microbiology, 2002; National Academy of Sciences, 2004; The Academy of Medicine, Engineering, and Science of Texas, 2004; Wilbur Cross Medal, Yale University, 2013.